Sudden integrated increases in biological Information.
We know that there must be sudden integrated increases in information from the nature of the systems that we have discovered operate within a cell.
All life as we know it operates in membrane bound units. This means that the first membrane bound cell had to occur at a specific moment when the membrane sealed on a blob of protoplasm. For this object to be really alive it needed
(a) All the information to code for the apparatus to replicate its own information
(b) All the information to gain sufficient energy to power replication of the information and the doubling of the structural molecules is needed.
(c) All the information to produce the machinery to double all the structural materials of that first living cell.
It is impossible to have this happening without a sudden increase in integrated information.
The origin of a new protein system from scratch.
If we imagine a tree of ancestry for all proteins then there is a limit to the degree with which new protein can be thought of as deriving from other proteins. A large number of proteins must be present to make the simplest living organism but there are large numbers of families of proteins which are additional to those which would have been derived from those necessary for the origin of life.
My understanding is that it is this clustering of interdependent information which is at the heart of Behe’s argument in Darwin’s Black Box and which is the insight which is at the heart of the biological ID debate.